The Effect of Dialysis Purification of Humic Substances Solutions on Mitotic Index

Alexander Popov¹, Didem Gyulter¹, Andrew Vishnaykov², Alexey Rusakov¹, Oleg Romanov¹, and Natalia

Kovaleva¹

1. Institute of Earth Sciences, St. Petersburg State University, St. Petersburg, Russian Federation

2. Biological Faculty, St. Petersburg State University, St. Petersburg, Russian Federation

Abstract: The biological activity of neutralized pyrophosphate solutions of humic substances and the same solutions that were purified by dialysis was compared. It was found that neutralized pyrophosphate solutions of humic substances (0.001 mg C per 1 ml) and their dialyzed solutions much increased the mitotic index in the apical root cells and onions (Allium cepa L.) and peas (Pisum sativum L.). Dialysis of neutralized pyrophosphate solutions of humic substances had no significant effect on the size of the mitotic index of the apical root cells of both plants.

Key words: humic substances, mitotic index, purification by dialysis, Allium cepa L., Pisum sativum L.